ITGAL anticorps

N° du produit ABIN120693

Cet anticorps anti-ITGAL est un anticorps Souris Monoclonal détectant ITGAL dans FACS, IP et Func. Adapté pour Humain. Ce Primary Antibody a été cité dans 3+ publications.

Aperçu rapide pour ITGAL anticorps (ABIN120693)

Antigène: ITGAL (Integrin alpha-L (ITGAL))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGAL est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Clone: MEM-83

Détail du produit anti-ITGAL anticorps

Specificité: The antibody recognizes an unique epitope on CD11a antigen (LFA-1 alpha chain) implicated in activation of LFA-1 complex.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogène: Human peripheral blood lymphocytes

Isotype: IgG1

Information d'application

Indications d'application: Functional Application. The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the I domain of the LFA-1 a subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1. Flow Cytometry. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 4-8 °C for one month or at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Références pour anticorps anti-ITGAL (ABIN120693)

Devine, Thakral, Nag, Dobbins, Hodsdon, Kavathas: "Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 6, pp. 3930-8, (2006) (PubMed).

Landis, McDowall, Holness, Littler, Simmons, Hogg: "Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3." dans: The Journal of cell biology, Vol. 126, Issue 2, pp. 529-37, (1994) (PubMed).

Landis, Bennett, Hogg: "A novel LFA-1 activation epitope maps to the I domain." dans: The Journal of cell biology, Vol. 120, Issue 6, pp. 1519-27, (1993) (PubMed).

Détails sur ITGAL

Antigène: ITGAL (Integrin alpha-L (ITGAL))

Autre désignation: CD11a / ITGAL

Sujet: CD11a (LFA-1a) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the aLb2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.Synonyms: CD11 antigen-like family member A, Integrin alpha-L, LFA-1, LFA1, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain

ID gène: 3683

UniProt: P20701

Pathways: Activated T Cell Proliferation, Integrin Complex